Journal: Oncotarget

Article Title: Prostate cancer diagnosis using epigenetic biomarkers, 3D high-content imaging and probabilistic cell-by-cell classifiers

doi: 10.18632/oncotarget.18985

Figure Lengend Snippet: Imaging was performed to acquire four categories of tissue frames corresponding to a sampling spectrum of epithelial and stromal compartments: epithelia only (E), epithelia with minor bordering stroma (E+s), mixed epithelia and stroma at various ratios (ES), and stroma only (S).

Article Snippet: Cultured cells included primary human prostate epithelial cells (HPrEpiC, ScienCell, Carlsbad, CA) as normal primary cells, and LNCaP (American Type Culture Collection, Manassas, VA), an androgen-sensitive prostate cancer cell line.

Techniques: Imaging, Sampling

Journal: Oncotarget

Article Title: Prostate cancer diagnosis using epigenetic biomarkers, 3D high-content imaging and probabilistic cell-by-cell classifiers

doi: 10.18632/oncotarget.18985

Figure Lengend Snippet: Differential levels and cellular heterogeneity of Biomarkers I and II between biopsied benign and cancerous prostatic tissues (represented by AC with GS6), as visualized by confocal scanning microscopy (A). Each marker (false-colored) was recorded in a separate channel. For each tissue sample all channels —including the multi-color overlay image— are presented as maximum intensity projections. (B) Quantitative presentation of biomarker levels as bar plots indicate for Biomarkers I: significant increase in DNA content (DAPI) and AMACR levels and simultaneous loss of the two epigenetic DNA modifications 5mC and 5hmC in both basal and luminal epithelial cells in AC versus benign tissue; luminal cells seemingly exhibit a stronger loss of 5hmC compared to basal cells. Biomarkers II: concurrent loss of suppressive chromatin-state marker H3K27me3 and decrease of chromatin-associated SAFB levels, while H3K9me3 and nuclear AR levels are highly elevated in AC versus benign tissue. Scale bar is 10 μm.

Article Snippet: Cultured cells included primary human prostate epithelial cells (HPrEpiC, ScienCell, Carlsbad, CA) as normal primary cells, and LNCaP (American Type Culture Collection, Manassas, VA), an androgen-sensitive prostate cancer cell line.

Techniques: Microscopy, Marker, Biomarker Discovery

Journal: Oncotarget

Article Title: Prostate cancer diagnosis using epigenetic biomarkers, 3D high-content imaging and probabilistic cell-by-cell classifiers

doi: 10.18632/oncotarget.18985

Figure Lengend Snippet: The data was separated into subsets representing the first biopsy (blue), the second biopsy (red), and finally prostatectomy (green). Each dot represents one cell. The results show a high overlap when epithelial and stromal compartments are analyzed together (ES). The overlap is reduced in the case of only a minor involvement of stroma (E+s). The best segregation is seen when the epithelial compartment is analyzed by itself, indicating the highest change (variance) for the analyzed markers. The latter subdata is missing data from two biopsies, as most patients for which epithelial (E) compartments could be analyzed were initially diagnosed with lots of AC and thus only underwent one biopsy prior to prostatectomy.

Article Snippet: Cultured cells included primary human prostate epithelial cells (HPrEpiC, ScienCell, Carlsbad, CA) as normal primary cells, and LNCaP (American Type Culture Collection, Manassas, VA), an androgen-sensitive prostate cancer cell line.

Techniques:

Journal: Oncotarget

Article Title: Prostate cancer diagnosis using epigenetic biomarkers, 3D high-content imaging and probabilistic cell-by-cell classifiers

doi: 10.18632/oncotarget.18985

Figure Lengend Snippet: Performance of Logistic regression model with the development data set and Biomarkers I, utilizing epithelial cell only (A) and all subsets of all imaged cells (B). 5mC shows best performance as single marker in both cases, and is only exceeded by the combined marker panel.

Article Snippet: Cultured cells included primary human prostate epithelial cells (HPrEpiC, ScienCell, Carlsbad, CA) as normal primary cells, and LNCaP (American Type Culture Collection, Manassas, VA), an androgen-sensitive prostate cancer cell line.

Techniques: Marker

Journal: Oncotarget

Article Title: Prostate cancer diagnosis using epigenetic biomarkers, 3D high-content imaging and probabilistic cell-by-cell classifiers

doi: 10.18632/oncotarget.18985

Figure Lengend Snippet: Logistic regression model coefficients for epithelial cells only

Article Snippet: Cultured cells included primary human prostate epithelial cells (HPrEpiC, ScienCell, Carlsbad, CA) as normal primary cells, and LNCaP (American Type Culture Collection, Manassas, VA), an androgen-sensitive prostate cancer cell line.

Techniques:

Journal: Oncotarget

Article Title: Prostate cancer diagnosis using epigenetic biomarkers, 3D high-content imaging and probabilistic cell-by-cell classifiers

doi: 10.18632/oncotarget.18985

Figure Lengend Snippet: Predictions of the logistic model based on epithelial cells only

Article Snippet: Cultured cells included primary human prostate epithelial cells (HPrEpiC, ScienCell, Carlsbad, CA) as normal primary cells, and LNCaP (American Type Culture Collection, Manassas, VA), an androgen-sensitive prostate cancer cell line.

Techniques:

Journal: Oncotarget

Article Title: Prostate cancer diagnosis using epigenetic biomarkers, 3D high-content imaging and probabilistic cell-by-cell classifiers

doi: 10.18632/oncotarget.18985

Figure Lengend Snippet: Validation of KNN classification for predicting tissue pathological categories (including cancer stages) using epithelial cells only and Biomarkers I

Article Snippet: Cultured cells included primary human prostate epithelial cells (HPrEpiC, ScienCell, Carlsbad, CA) as normal primary cells, and LNCaP (American Type Culture Collection, Manassas, VA), an androgen-sensitive prostate cancer cell line.

Techniques: Biomarker Discovery

Journal: Oncotarget

Article Title: Prostate cancer diagnosis using epigenetic biomarkers, 3D high-content imaging and probabilistic cell-by-cell classifiers

doi: 10.18632/oncotarget.18985

Figure Lengend Snippet: Validation of KNN classification for predicting GS based on epithelial cells only with Biomarkers I

Article Snippet: Cultured cells included primary human prostate epithelial cells (HPrEpiC, ScienCell, Carlsbad, CA) as normal primary cells, and LNCaP (American Type Culture Collection, Manassas, VA), an androgen-sensitive prostate cancer cell line.

Techniques: Biomarker Discovery

Journal: Nutrition research and practice

Article Title: Synergistic anticancer activity of resveratrol in combination with docetaxel in prostate carcinoma cells.

doi: 10.4162/nrp.2021.15.1.12

Figure Lengend Snippet: Fig. 3. Expression of apoptosis- and necroptosis-related proteins by RSV and DTX in LNCaP and HPrECs. (A) Cells were treated with increasing concentrations of RSV (0, 10, 20, and 30 μM) and DTX (0, 1, 2, and 3 nM) for 48 h. (B) Cells were treated with RSV (20 μM) and DTX (2 nM) alone or in combination for 48 h. The levels of apoptosis- and necroptosis-related proteins were assessed by Western blotting. RSV, resveratrol; DTX, docetaxel; Bcl-2, B-cell lymphoma 2; BAX, Bcl-2-associated X protein; PARP, poly ADP-ribose polymerase; RIP3, receptor-interacting protein kinase-3; MLKL, mixed lineage kinase domain-like protein; HPrEC, human prostate epithelial cell.

Article Snippet: LNCaP (human prostate cancer) and human prostate epithelial cell (HPrEC) lines were acquired from the American Type Culture Collection (ATCC; Manassas, USA).

Techniques: Expressing, Western Blot

Journal: Cancer Research

Article Title: Maspin Retards Cell Detachment via a Novel Interaction with the Urokinase-Type Plasminogen Activator/Urokinase-Type Plasminogen Activator Receptor System

doi: 10.1158/0008-5472.can-05-3514

Figure Lengend Snippet: Figure 1. Maspin inhibits cell migration without affecting cell growth. A, maspin inhibits cell scattering but not cell growth. a, growth curves of parental DU145 cells (o), mock-transfected Neo cells (5), and three maspin-transfected cell lines M3 (.), M7 (!), and M10 (n); b, representative phase-contrast microscopic images of Neo, M3, M7, and M10 clonal cells in anchorage-dependent colonogenic assay. Magnification, 200. B, maspin reexpression inhibits DU145 cell detachment. a, post-detachment measurement of adherent cells by sulforhodamine B assay (A 550). b, representative microscopic images of sulforhodamine B–stained cells at 0 or 2 hours into the detachment treatment. Magnification, 200. C, adenoviral expression of maspin inhibits cell detachment. a, Western blot of maspin in the lysates of cells that had been infected with Ad-CMV or Ad-CMV-mas for the indicated post-infection (p.i.) time periods. A total of 30 Ag of cell lysate proteins was loaded into each lane. Western blot of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) on the same membrane was done to normalize the protein loading. b, sulforhodamine B assay (A550) of post-detachment adherent DU145 cells that had been infected for 3 days with Ad-CMV or Ad-CMV-mas. D, a, dose-dependent effect of rMaspin on DU145 cell detachment. Post-detachment adherence was quantified by sulforhodamine B assay and normalized by the BSA background at the corresponding concentration. b, Western blot/densitometric quantification of maspin secretion by CRL2220. Concentrated culture media (CM) conditioned by 1 106 cells for the indicated periods of time along with purified rMaspin standard were subjected to denaturing SDS-PAGE and Western blot of maspin. Points/columns, average of three repeats; bars, SE. The error bars are omitted in (A, a) for clarity.

Article Snippet: Normal human prostate epithelial cells CRL2220 (American Type Culture Collection, Manassas, VA) were cultured in serum-free keratinocyte growth medium (KGM-SF).

Techniques: Migration, Transfection, Sulforhodamine B Assay, Staining, Expressing, Western Blot, Infection, Membrane, Concentration Assay, Purification, SDS Page

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Nanoparticle decoration impacts airborne fungal pathobiology

doi: 10.1073/pnas.1804542115

Figure Lengend Snippet: Pathobiological impact of NP-conidia complex formation. (A) NP coating increases resistance against defensins, HNP-1, or hbD3. Conidia were exposed to defensins, and their minimal inhibitory concentration (MIC) was determined by the AlamarBlue assay. (B) Binding of ZnO NP to conidia reduced NP toxicity. HPECs were cultivated at the ALI and exposed to aerosol containing either ZnO or ZnO-conidia complexes. Cell vitality was assessed after 6 h. (C) NP coating increases IL-1β secretion. THP-1M cells were exposed to SiOR, conidia, or SiOR-conidia complexes for 90 min. IL-1β was analyzed by ELISA. (D–F) NP coating inhibits TLR2-dependent uptake by phagocytes. (D) Fluorescence microscopy showing internalization of NP-conidia complexes into primary human monocytes and neutrophils. (Scale bar: 10 µm.) (E) Automated microscopy demonstrating reduced internalization of SiOR-conidia complexes into THP-1M macrophages. (F) Reduced internalization of NP-coated conidia in murine neutrophils from wild-type, but not TLR2−/−-deficient, animals. (G) Reduced internalization of NP-coated conidia in THP-1M cells increases fungal burden (CFU). *P = 0.05; **P = 0.01.

Article Snippet: Primary HPECs cells were purchased from Provitro AG and cultured for up to five passages in Alveolar Epithelial Cell Medium (Provitro AG) as described previously ( 35 , 36 ).

Techniques: Concentration Assay, Alamar Blue Assay, Binding Assay, Enzyme-linked Immunosorbent Assay, Fluorescence, Microscopy

Journal: Antioxidants

Article Title: Effects of Drying Methods on Antioxidant, Anti-Inflammatory, and Anticancer Potentials of Phenolic Acids in Lovage Elicited by Jasmonic Acid and Yeast Extract

doi: 10.3390/antiox10050662

Figure Lengend Snippet: Pearson’s correlation coefficients for phenolic acid content and in vitro bioactivities.

Article Snippet: The PBS extracts of the control and elicited lovage leaves dried with all of the tested methods in the tested concentrations (0.1–20 mg dw/mL) had no effect on the number of cells (in MTT and NR assays), viability (no impact on apoptosis or necrosis), or percentages of cell cycle phases (G1, S, or G2) in the gastric cancer NCI-N87 (ATCC ® CRL5822 TM), prostate cancer VCaP (ATCC ® CRL-2876 TM), and healthy prostate HPrEC (ATCC ® PCS-440-010 TM) cell lines, as compared to cells that were not exposed to the extracts ( ).

Techniques: In Vitro, Inhibition

Journal: Antioxidants

Article Title: Effects of Drying Methods on Antioxidant, Anti-Inflammatory, and Anticancer Potentials of Phenolic Acids in Lovage Elicited by Jasmonic Acid and Yeast Extract

doi: 10.3390/antiox10050662

Figure Lengend Snippet: In vitro effects of the highest concentration of the analyzed extracts after 72-h treatment on the cancer gastric epithelial NCI-N87 (ATCC ® CRL5822 ™), prostate cancer VCaP (ATCC ® CRL-2876 ™), and healthy prostate epithelial HPrEC cell lines (ATCC ® PCS-440-010™).

Article Snippet: The PBS extracts of the control and elicited lovage leaves dried with all of the tested methods in the tested concentrations (0.1–20 mg dw/mL) had no effect on the number of cells (in MTT and NR assays), viability (no impact on apoptosis or necrosis), or percentages of cell cycle phases (G1, S, or G2) in the gastric cancer NCI-N87 (ATCC ® CRL5822 TM), prostate cancer VCaP (ATCC ® CRL-2876 TM), and healthy prostate HPrEC (ATCC ® PCS-440-010 TM) cell lines, as compared to cells that were not exposed to the extracts ( ).

Techniques: In Vitro, Concentration Assay